the fpg kit is tailored for users whowant to combine the twistdx amplification technology with analternative twistdx reporter probe system, fluorescenttwistamp® fpgprobe, in a homogenous format.
our newformulation kit includes extra ssdna binding protein and dntp toimprove rpa performance. we highly recommend customers switch tothis formulation as we expect improved performance for mostuses.
features:
newimproved formulation
real-timeprobe detection
single-molecule detection
twista®-compatible
multiplexable
1 x box of96 reactions
pleasenote:
formagnetic mixing using the t16 device, bearing balls may be added toreactions at any time before the reaction is initiated withmg.
theseprobes have fewer design constraints thantwistamp®exoprobe, although kinetics of fluorescence accumulation may beslower. in addition to the basic components it contains a powerfulnuclease (fpg) which will process twistamp fpg probe during theamplification reaction itself and generate a real-time read-out.the presence of fpg will not reduce the final overall yield ofamplified material in contrast to the use of exonuclease iii in thetwistamp®exokit allowing end point gel analysis, but the kinetics of probecutting are sometimes slower than with the exo system.
there are96 reactions in each kit.
TwistDX,fpg,TAFPG01kit_T,TwistAmp fpg