产品描述：
twistamp®核酸扩增产品，能够在15分钟内进行常温下的单分子核酸检测该技术对硬件设备的要求很低，特别适合用于体外诊断、兽医、食品安全、生物安全、农业等领域。twistampexo rt荧光检测探针法rt-rpa. 除基本组分外,外加通用的逆转录酶，但不含rnase抑制剂。
basic information
rpa
1) primers must be 30-35 bases
2) works best at constant temperature(40°c)
3) amplicons of 80-400bp are preferred
4) twistamp® exo probe required - seeoverleaf
set-up (single-plex)1
1) prepare reaction mix in 1.5ml tube:
primer a (10μm) 2.1μl
primer b (10μm) 2.1μl
twistamp® exo probe (10μm) 0.6μl
rehydration buffer 29.5 μl
template,
rnase inhibitor and dh2o 13.2μl
(total volume 47.5 μl)
2) add reaction mix to freeze-dried reaction. pipette tomix.
3) add 2.5 μl of 280mm mgac (supplied) and mix well to startreaction.
4) place strip in twista® and start run: 40°c, 20minutes.
5) remove strip after 5-7 minutes, invert vigorously 8-10 times tomix & spin briefly,replace in twista.
warning: rpa reactions start at room temperature as soonas
magnesium is added.