tabsrt01kit | twistamp™ basicrt | 用于逆转录rpa反应 |
产品描述：
twistamp®核酸扩增产品，能够在15分钟内进行常温下的单分子核酸检测该技术对硬件设备的要求很低，特别适合用于体外诊断、兽医、食品安全、生物安全、农业等领域。twistampbasic用于逆转录反应，cdna快速扩增. 除基本组分外,还加有通用的逆转录酶，但不含rnase抑制剂。
basic information
rpa
1) primers must be 30-35 bases
2) works best at constant temperature(40-42°c)
3) amplicons of 80-400bp are
set-up (single-plex)1
1) prepare reaction mix in 1.5ml tube:
primer a (10μm) 2.4 μl
primer b (10μm) 2.4 μl
rehydration buffer 29.5 μl
template,
rnase inhibitor and dh2o 13.2 μl
(total volume 47.5 μl)
vortex and spin briefly
2) add reaction mix to freeze-dried reaction. pipette tomix.
3) add 2.5 μl of 280mm mgac (supplied) and mix well to startreaction.
4) incubate at 40-42°c for 20-40 minutes.
4b) for low template copy number,
remove strip after 5-7 minutes, invert
vigorously 8-10 times to mix & spin
briefly, replace in heating device.
5) after 20-40 minutes, clean amplicons before running on agarosegels.
warning: rpa reactions start at room temperature as soonas
magnesium is added.
TwistDX，Basic RT，TABSRT01KIT，TwistAmp Basic RT