TwistAmp exo试剂盒

产品描述：

twistamp®核酸扩增产品，能够在15分钟内进行常温下的单分子核酸检测该技术对硬件设备的要求很低，特别适合用于体外诊断、兽医、食品安全、生物安全、农业等领域。twistampexo 用于real-time探针检测. 除基本组分外,还含有内切酶3，减少扩增产物的末端拷贝量，所以不能用于电泳分析，但在rpa系统中产生更强的动态荧光信号，便于实时定量。

basic information

rpa

1) primers must be 30-35 bases

2) works best at constant temperature (37-39°c)

3) amplicons of 80-400bp are preferred

4) twistamp® exo probe required - see overleaf

set-up (single-plex)1

1) prepare reaction mix in 1.5ml tube:

primer a (10μm)    2.1 μl

primer b (10μm)    2.1 μl

twistamp® exo probe (10μm)    0.6 μl

rehydration buffer   29.5 μl

template and dh2o   13.2 μl

(total volume   47.5 μl)

vortex and spin briefly

2) add reaction mix to freeze-dried reaction. pipette tomix.

3) add 2.5 μl of 280mm mgac (supplied) and mix well to startreaction.

4) place strip in twista® and start run: 37-39°c, 20minutes.

4b) for low template copy number, remove strip after 4 minutes,vortex &

spin briefly, replace in twista®.

warning: rpa reactions start at room temperature as soonas