locus pet-3a(+) 4640 bp dna syn
definition pet-3a
comment this file is created by vectornti
https://www.biofeng.com/
comment origdb|genbank
comment vntauthorname|biofeng.com|
comment vntoauthorad1|novageninc.|
organism other se; artificial se;vectors.
features location/
source 1..4640
/organism="pet-3a"
/mol_type="other dna"
gene 86..1553
/label="tet (w/ gaps)"
/gene="tet (w/ gaps)"
terminator complement(379..507)
/label="t7_terminator"
misc_feature 447..465
/label="t7_terminal_primer"
misc_feature complement(519..551)
/label="t7_leader"
misc_feature complement(534..552)
/label="xpress_fwd_primer"
misc_feature complement(560..576)
/label="t7_transl_en_rbs"
promoter complement(613..631)
/label="t7_promoter"
misc_feature complement(715..734)
/label="pbrrevbam_primer"
cds 768..1553
/label="orf frame 3"
cds complement(900..1358)
/label="orf frame 1"
misc_feature 2192..2383
/label="rop"
misc_feature complement(2377..2399)
/label="pgex_3_primer"
gene complement(3572..4432)
/label="ampicillin"
/gene="ampicillin"
cds complement(3572..4432)
/label="orf frame 2"
promoter complement(4474..4502)
/label="ampr_promoter"
origin
1 ttctcatgtt tgacagctta tcatcgataa gctttaatgcggtagtttat cacagttaaa
61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaatgcgctcatcg tcatcctcgg
121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgccgggcctctt
181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgctagcgctata
241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgaccgctttggccg
301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcgatcatggcgac
361 cacacccgtc ctgtggatat ccggatatag ttcctccttt cagcaaaaaacccctcaaga
421 cccgtttaga ggccccaagg ggttatgcta gttattgctc agcggtggcagcagccaact
481 cagcttcctt tcgggctttg ttagcagccg gatccgcgac ccatttgctgtccaccagtc
541 atgctagcca tatgtatatc tccttcttaa agttaaacaa aattatttctagagggaaac
601 cgttgtggtc tccctatagt gagtcgtatt aatttcgcgg gatcgagatctcgatcctct
661 acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgctggcgcctata
721 tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatgagcgcttgtt
781 tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgccatctccttgc
841 atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactgggctgcttcc
901 taatgcagga gtcgcataag ggagagcgtc gaccgatgcc cttgagagccttcaacccag
961 tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatgactgtcttct
1021 ttatcatgca actcgtagga caggtgccgg cagcgctctg ggtcattttcggcgaggacc
1081 gctttcgctg gagcgcgacg atgatcggcc tgtcgcttgc ggtattcggaatcttgcacg
1141 ccctcgctca agccttcgtc actggtcccg ccaccaaacg tttcggcgagaagcaggcca
1201 ttatcgccgg catggcggcc gacgcgctgg gctacgtctt gctggcgttcgcgacgcgag
1261 gctggatggc cttccccatt atgattcttc tcgcttccgg cggcatcgggatgcccgcgt
1321 tgcaggccat gctgtccagg caggtagatg acgaccatca gggacagcttcaaggatcgc
1381 tcgcggctct taccagccta acttcgatca ctggaccgct gatcgtcacggcgatttatg
1441 ccgcctcggc gagcacatgg aacgggttgg catggattgt aggcgccgccctataccttg
1501 tctgcctccc cgcgttgcgt cgcggtgcat ggagccgggc cacctcgacctgaatggaag
1561 ccggcggcac ctcgctaacg gattcaccac tccaagaatt ggagccaatcaattcttgcg
1621 gagaactgtg aatgcgcaaa ccaacccttg gcagaacata tccatcgcgtccgccatctc
1681 cagcagccgc acgcggcgca tctcgggcag cgttgggtcc tggccacgggtgcgcatgat
1741 cgtgctcctg tcgttgagga cccggctagg ctggcggggt tgccttactggttagcagaa
1801 tgaatcaccg atacgcgagc gaacgtgaag cgactgctgc tgcaaaacgtctgcgacctg
1861 agcaacaaca tgaatggtct tcggtttccg tgtttcgtaa agtctggaaacgcggaagtc
1921 agcgccctgc accattatgt tccggatctg catcgcagga tgctgctggctaccctgtgg
1981 aacacctaca tctgtattaa cgaagcgctg gcattgaccc tgagtgatttttctctggtc
2041 ccgccgcatc cataccgcca gttgtttacc ctcacaacgt tccagtaaccgggcatgttc
2101 atcatcagta acccgtatcg tgagcatcct ctctcgtttc atcggtatcattacccccat
2161 gaacagaaat cccccttaca cggaggcatc agtgaccaaa caggaaaaaaccgcccttaa
2221 catggcccgc tttatcagaa gccagacatt aacgcttctg gagaaactcaacgagctgga
2281 cgcggatgaa caggcagaca tctgtgaatc gcttcacgac cacgctgatgagctttaccg
2341 cagctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgcagctcccgga
2401 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtcagggcgcgtc
2461 agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcgatagcggagt
2521 gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgcaccatatatgc
2581 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgctcttccgctt
2641 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggtatcagctcact
2701 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaagaacatgtgag
2761 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcgtttttccata
2821 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagaggtggcgaaacc
2881 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtgcgctctcctg
2941 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcgggaagcgtggcgc
3001 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgctccaagctgg
3061 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggtaactatcgtc
3121 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccactggtaacagga
3181 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtggcctaactacg
3241 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagttaccttcggaa
3301 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggtggtttttttg
3361 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcctttgatctttt
3421 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttggtcatgagat
3481 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagttttaaatcaatct
3541 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagtgaggcaccta
3601 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtcgtgtagataa
3661 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccgcgagacccac
3721 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggccgagcgcagaa
3781 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgggaagctagag
3841 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctgcaggcatcgtgg
3901 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacgatcaaggcgag
3961 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcctccgatcgttg
4021 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactgcataattctc
4081 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactcaaccaagtcat
4141 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaacacgggataata
4201 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttcttcggggcgaa
4261 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccactcgtgcaccca
4321 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaaacaggaaggc
4381 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactcatactcttcc
4441 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcggatacatatttg
4501 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccgaaaagtgccac
4561 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaataggcgtatcacga
4621 ggccctttcg tcttcaagaa
//