prl家族是野生型海肾萤光素酶(rluc) 报告基因对照载体。prl 载体提供组成型表达的海肾萤光素酶，可与一个萤火虫萤光素酶载体联合共转染哺乳动物细胞。海肾萤光素酶的表达为实验的萤火虫萤光素酶报告基因正态化提供内对照值。prl 载体包含一个编码海肾萤光素酶(rluc) 的cdna, 这个cdna 从珊瑚虫类腔肠动物海肾(renilla reniformis)中克隆而来。目前 有四个不同的启动子构造。其中hsv- 胸腺嘧啶核苷激酶启动子(prl-tk) 相对较弱，更适用于海肾萤光素酶报告基因载体 的中性组成型表达。早期sv40 增强子/ 启动子区域(prl-sv40) 和cmv 极早期增强子/启动子区域(prl-cmv)一般提供高水平转录，因此可能不适于作为实验载体带有强调控元件的共报告基因。一般我们建议在将要应用的目标细胞中验证某一个特定共报告基因的性能。除了经过修饰的rluc 报告基因外，所有prl载体均是从dam–/dcm– e. coli k 宿主菌株分离而来，这样可以使用对dam 或 dcm 甲基化敏感的限制性内切酶进行消化。
locus prl-tk 4045 bp dna syndefinition prl-tkaccession keywords source organism other se; artificial se; vectors.features location/ source 1..4045 /organism="prl-tk" /mol_type="other dna" cds 332..796 /label="orf frame 2" promoter complement(532..680) /label="tata_promoter" promoter 543..758 /label="tk_promoter" misc_feature 829..961 /label="chim_int" promoter 1006..1024 /label="t7_promoter" cds 1034..1969 /label="orf frame 2" terminator complement(1997..2116) /label="sv40_pa_terminator" misc_feature complement(2011..2030) /label="ebv_rev_primer" promoter 2289..2317 /label="ampr_promoter" gene 2359..3219 /label="ampicillin" /gene="ampicillin" cds 2359..3219 /label="orf frame 1" rep_origin 3374..3993 /label="pbr322_origin"origin 1 agatctaaat gagtcttcgg acctcgcggg ggccgcttaa gcggtggtta gggtttgtct 61 gacgcggggg gagggggaag gaacgaaaca ctctcattcg gaggcggctc ggggtttggt 121 cttggtggcc acgggcacgc agaagagcgc cgcgatcctc ttaagcaccc ccccgccctc 181 cgtggaggcg ggggtttggt cggcgggtgg taactggcgg gccgctgact cgggcgggtc 241 gcgcgcccca gagtgtgacc ttttcggtct gctcgcagac ccccgggcgg cgccgccgcg 301 gcggcgacgg gctcgctggg tcctaggctc catggggacc gtatacgtgg acaggctctg 361 gagcatccgc acgactgcgg tgatattacc ggagaccttc tgcgggacga gccgggtcac 421 gcggctgacg cggagcgtcc gttgggcgac aaacaccagg acggggcaca ggtacactat 481 cttgtcaccc ggaggcgcga gggactgcag gagcttcagg gagtggcgca gctgcttcat 541 ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg gaagaaatat atttgcatgt 601 ctttagttct atgatgacac aaaccccgcc cagcgtcttg tcattggcga attcgaacac 661 gcagatgcag tcggggcggc gcggtcccag gtccacttcg catattaagg tgacgcgtgt 721 ggcctcgaac accgagcgac cctgcagcga cccgcttaaa agcttgattc ttctgacaca 781 acagtctcga acttaagctg cagaagttgg tcgtgaggca ctgggcaggt aagtatcaag 841 gttacaagac aggtttaagg agaccaatag aaactgggct tgtcgagaca gagaagactc 901 ttgcgtttct gataggcacc tattggtctt actgacatcc actttgcctt tctctccaca 961 ggtgtccact cccagttcaa ttacagctct taaggctaga gtacttaata cgactcacta 1021 taggctagcc accatgactt cgaaagttta tgatccagaa caaaggaaac ggatgataac 1081 tggtccgcag tggtgggcca gatgtaaaca aatgaatgtt cttgattcat ttattaatta 1141 ttatgattca gaaaaacatg cagaaaatgc tgttattttt ttacatggta acgcggcctc 1201 ttcttattta tggcgacatg ttgtgccaca tattgagcca gtagcgcggt gtattatacc 1261 agaccttatt ggtatgggca aatcaggcaa atctggtaat ggttcttata ggttacttga 1321 tcattacaaa tatcttactg catggtttga acttcttaat ttaccaaaga agatcatttt 1381 tgtcggccat gattggggtg cttgtttggc atttcattat agctatgagc atcaagataa 1441 gatcaaagca atagttcacg ctgaaagtgt agtagatgtg attgaatcat gggatgaatg 1501 gcctgatatt gaagaagata ttgcgttgat caaatctgaa gaaggagaaa aaatggtttt 1561 ggagaataac ttcttcgtgg aaaccatgtt gccatcaaaa atcatgagaa agttagaacc 1621 agaagaattt gcagcatatc ttgaaccatt caaagagaaa ggtgaagttc gtcgtccaac 1681 attatcatgg cctcgtgaaa tcccgttagt aaaaggtggt aaacctgacg ttgtacaaat 1741 tgttaggaat tataatgctt atctacgtgc aagtgatgat ttaccaaaaa tgtttattga 1801 atcggaccca ggattctttt ccaatgctat tgttgaaggt gccaagaagt ttcctaatac 1861 tgaatttgtc aaagtaaaag gtcttcattt ttcgcaagaa gatgcacctg atgaaatggg 1921 aaaatatatc aaatcgttcg ttgagcgagt tctcaaaaat gaacaataat tctagagcgg 1981 ccgcttcgag cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg 2041 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt 2101 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag 2161 ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg taaaatcgat 2221 aaggatccag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc 2281 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa 2341 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt 2401 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct 2461 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc 2521 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta 2581 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac 2641 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc 2701 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac 2761 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg 2821 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac 2881 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc 2941 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt 3001 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga 3061 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc 3121 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag 3181 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca 3241 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc 3301 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca 3361 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc 3421 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta 3481 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt 3541 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc 3601 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg 3661 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg 3721 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag 3781 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc 3841 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat 3901 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg 3961 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc 4021 tggccttttg ctcacatggc tcgac//更多请点击：https://www.miaolingbio.com/cn/product/iphone/577.html