主要组成成分lapro taq dna polymerase(e00013)性状试剂适应症科研试验规格1000u生产企业genscript药（械）准字genscript e00013

dna polymerase(e00013)

产品价格：

cat.no.namesizeprice(¥)edna polymerase100iu149.00edna polymerase250iu260.00

产品说明：

laprota是具有5"→3"dna聚合活性和3"→5"校对活性的dna聚合酶，可用于高效扩增长片段的复杂模板。laprota在75℃（其反应适温度）时，扩增产物的率是普通ta的10倍以上，可扩增长达17.5kb来自于人genomicdna或来自于λdna的长达20kb的dna片段。使用laprota扩增长片段dna模板时，可有效减少拖尾效应，去除背景影响，扩增产物有3"端有一个a碱基，可以直接用于t/a克隆。

产品用途：

ta克隆片段分析位点特异性突变

产品成分：

dna polymerase
1.5ml×2 2x buffer，500mm tris-hcl (ph8.9, 25℃)，0.5% tween 20，0.5%nonidet p40，1mg/ml bsa，3mm mgcl2

活性：

高保真pcr扩增，错配率只有2×10-6。没有5"→3"外切酶活性和3"→5"校对活性，具有ta克隆所需的extendase活性。

单位定义：

1单位(u)dna polymerase活力定义为在74℃条件下，30分钟内，以活性化的大马哈鱼精子dna作为模板/引物，催化10nmol脱氧核苷酸掺入反应成为酸不溶性物质所需的酶量。

储存缓存液及浓度：

储存于含有50mm tris hcl (ph8.1)，0.1mm edta，1mm dtt，0.1% (v/v) nonidetp40, 0.1% (v/v) tween 20和50% (v/v)甘油的缓冲液，5units/μl。

储存条件：

-20℃

应用举例：

1. shown below is a pcr amplification of 1kb to 20kb fragmentsfrom λdna using dna polymerase.
figure 1. pcr amplification of 1-20kb fragments from λdna usingdna polymerase. 1u of enzyme was used for each50μl pcr. 5μl of pcr mixture was analyzed in each lane. lane1 showsa fragment of 1kb; lane2: 2kb; lane3: 3kb; lane4: 4kb; lane5: 5kb;lane6: 6kb; lane7: 8kb; lane8: 10kb; lane9: 12kb; lane10: 20kb.lanem shows genscript"s dna kb ladder (genscript, m101r).
2. shown below is a pcr amplification of 17.6kb fragments fromhuman genomic dna and 20kb fragments from λdna usingdna polymerase.
figure 2. pcr amplification of 17.6kb fragments (lanes2, 3, and 4)from human genomic dna and 20kb fragments (lanes6, 7, and 8) fromλdna using dna polymerase. all pcrs were performedin 50μl, and 3μl of reaction mixture was loaded in each lane.
lane1: 5udna polymerase was used to amplify17.6kb fragments from human genomic dna.
lanes2, 3 and 4: 1u, 2u, and 5u of dna polymerasewere used to amplify 17.6kb fragments from human genomic dna.
lane5: 5u kod dna polymerase was used to amplify 20kb fragmentsfrom λdna.
lanes6, 7 and 8: 1u, 2u and 5u of dna polymerasewere used to amplify 20kb fragment from λdna.