Oligo d(T) 18

主要组成成分oligo d(t) 18性状试剂适应症科研试验规格2od生产企业genscript药（械）准字genscript da0034nameselectedcloneez? pcr cloning kit

cloneez® pcr cloning kit

description

the genscriptcloneez®pcr cloningkitis designed for the quick pcr cloning , it isespecially powerful in high-throughput cloning of pcr products intoany destination vector effectively by sidestepping the tedious andlimiting tasks such as selecting proper restriction enzymes,phosphatases, or ligases. using our proprietarycloneez®enzyme, this kit is able to rapidly generateprecise and directional constructs by carrying out 30-minuteincubation at room temperature (20 °c-25 °c).

key features

the cloneez®pcr cloning kit offers flexible and cloning and enables users to::

clone into any vector without any need for restriction enzymes,phosphatase treatments, or ligasesprovide up to 12 kb pcr cloning at any restriction siterapidly and precisely generate correctly oriented constructsand insertswork with pcr that employ any thermostable polymerases
applicationsthe genscript cloneez®pcr cloning kit can be used in avariety of applications:pcr cloninghtp pcr cloningjoining of dna fragments
the inserts were detected by pcr screening using single colonies.some of the results are shown in the figure below.
technical manualdownload pdf filetm0279
componentscomponentspuc57 linearized (kpni/hindiii,100 ng/μl)1-kb controlinsert (100 ng/μl)10x cloneez?reactionbuffercloneez?enzyme (5 u/μl)l0033910 μl10 μl100 μl50 μl
documentmsds:20081029013928(pdf)