IDH2(R172G) 小鼠单抗/2022动态已更新/武汉费斯德生物
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- 武汉费斯德生物科技有限公司
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- ￥4800.00元每个
- 品牌
- NewEast Bio
- 货号
- 26321
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- 100微升
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- 027-63497286
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- 18162422647
- 联系人
- 付女士
- 所在地
- 武汉市江汉区前进五路97号5栋1层8号
- 更新时间
- 2022-11-25 16:23
| 货号: 26231 |
| 基因符号: idh2; d2hga2; icd-m; idh; idhm; idp; idpm;mnadp-idh |
| 描述: anti-idh2(r172g) mouse monoclonal antibody |
| 背景介绍: isocitrate dehydrogenase (idh) catalyze theoxidative decarboxylation of isocitrate to 2-oxoglutarate. theisocitrate and isopropylmalate dehydrogenases family has threemembers, idh1, idh2 and idh3. idh2 plays a role in intermediarymetabolism and energy production. defects in idh2 are the cause ofd-2-hydroxyglutaric aciduria type 2 (d2hga2). somatic mosaicmutations of this protein have also been found associated to ollierdisease and maffucci syndrome, and r172g idh2 mutations do exist indiffusely infiltrative gliomas. |
| 免疫原: a synthetic peptide from the internal region ofidh2 which includes the mutation of r172g, human origin |
| 经过测试的应用: elisa, wb, if, ihc |
| 推荐稀释比: elisa: 1: 1000-1: 5000 wb: 1: 100-1: 1000 if: 1: 50-1: 100 ihc: 1: 50-1: 100 |
| 浓度: 1 mg/ml |
| host: mouse |
| 形式: liquid |
| 克隆性: monoclonal |
| 亚型: igg |
| 纯度: purified from ascites |
| 防腐剂: no |
| 成分: pbs(without mg2+ andca2+),ph 7.4, 150 mm nacl, 50% glycerol |
| 种属反应性: recognizes r172g mutant, but not wild-type idh2of vertebrates |
| 储存条件: store at -20°c. avoid repeated freezingand thawing |
蛋白质印迹:
westernblot analysis of recombinant idh2(r172g) and wildtypeproteins.
purified his-tagged idh2(r172g) (lane 1) and correspondingwild-type idh2 protein (lane 2) were blotted with anti-idh2(r172g)monoclonal antibody (cat. #26231).
免疫荧光:
immunofluorescenceof cells expressing idh2 proteins with anti-idh2(r172g)antibody.
hek293t cells were transfected with pcdna3-gfp-idh2 (wt) plasmid(left column) or pcdna3-gfp-idh2(r172g) plasmid (right column),then fixed and stained with anti-idh2(r172g) monoclonal antibody(cat. #26231).
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