lucigen总代理中北林格 感受态细胞选择指南

北京中北林格供应lucigen总代理中北林格 感受态细胞选择指南，中北林格是biosearchtechnologies lucigen总代理，提供biosearchtechnologies lucigen产品货号报价查询。代理的其他品牌包括lucigen总代理，epicentre总代理，illumina总代理，icllab总代理，immunoreagents总代理。
blue-white capabl

phage t1 resistant

end a -/reca

restriction-deficient (rms)

clone methylated dna

inducible plasmid copy number

f’episome

antibiotic resistance

electrocompetent:efficiency

chemically competent:efficiency

常规克隆及文库构建

e. cloni® 10g (classic)

√

√

√

√

Ø

Ø

str

≥5 x109

106-109

e. cloni 10g (elite)

≥2 x1010

-

e. cloni 10g (supreme)

≥4 x1010

e.cloni 10g f’（elite)

e. cloni 5-alpha

Ø

-

-

≥1 x108

不稳定的插入物或有毒基因

copycutter™ epi400™

√

≥1 x1010

≥1 x107

大片段,plasmids和fosmids质粒

transformax™ ec100™

Ø

≥5 x108

transformax epi300™

a

transformax epi300™-t1r

bac克隆

bac-optimised replicator™ v2.0

amp, str

e. cloni bac-optimised 10g

bigeasy™-tsa

b

c

噬菌体展示

tg1

Ø

d

er2738

tet

ss320 (mc1061 f’)

e

tet, str

mc1061 f-

crispr sgrna文库及慢病毒质粒克隆

endura™

f

g

site-directed mutagenesis

cj236

cam

≥1 x109

 

amp, ampicillin; cam, chloramphenicol; str,streptomycin; tet,tetracycline a:requires oriv vectors (e.g., copyright® andpccfos)b: re: only with pjazz®vectorsd: mcrb- only; notsuitable for cloning eukaryotic genomicdnae: mcra- mcrb-; notsuitable for cloning eukaryotic genomicdnaf: reca13onlyg: mcrb- mrr-; notsuitable for cloning eukaryotic genomicdna 

 

primarypromoter

background expressioncontrol

lowendotoxin

antibioticresistance

electrocompetent:efficiency

chemicallycompetent:efficiency

常规蛋白表达

e. cloni expressbl21(de3)

t7

Ø

Ø

-

≥5 x 109

≥1 x 107

hl-control™bl21(de3)

t7

√

gen

-

hl-control™10g

plac,ptac,ptrc,t5lac,rhapbad

gen, str

≥1 x 109

clearcoli®bl21(de3)

　     t7

√

≥1 x 109

-

带毒性蛋白表达

overexpress™c41(de3)

≥1 × 109

≥1 × 106

overexpress c41(de3)plyss

√

cam

overexpressc43(de3)

overexpress c43(de3)plyss