CAS92-32-0 EINECS202-147-8化学式C17H19ClN2O 分子量302.8InChIInChI=1/C17H19N2O.ClH/c1-18(2)14-7-5-12-9-13-6-8-15(19(3)4)11-17(13)20-16(12)10-14;/h5-11H,1-4H3;1H/q+1;/p-1InChIKeyINCIMLINXXICKS-UHFFFAOYSA-M熔点250-260°C(lit.)水溶性Soluble in water. Also soluble in methyl cellosolve (9 mg/mL) andethanol (3 mg/mL).溶解度溶于水; 微溶于乙醇、乙二醇、甲基溶纤剂存储条件room temp稳定性稳定。与强氧化剂不相容。外观粉末/固体颜色PinkZui大波长(λmax)['548 nm']Merck13,8097BRN3795789MDL号MFCD00011725体外研究Pyronin Y forms fluorescent complexes with double-stranded nucleicacids, especially RNA, enabling semi- analysis ofcellular RNA in flow cytometry, to estimate the RNA content percell in formalin fixed EL4 leukosis tumor cells, enzyme dispersedR3327-G rat prostatic adenocarcinoma cells, mouse spleen cellsstimulated with concanavalin A, and human peripheral bloodlymphocytes stimulated with phytohemagglutinin. A fluorescentstaining procedure based on pyronin Y is described. Theuehas been used to stain RNA in human reticulocytes for subse analysis and sorting. In viable cells this dye alsoaccumulates in mitochondria. At a concentration of 1.7 to 3.3 μM,pyronin Y is localized almost exclusively in mitochondria ofcultured cells, similar to another mitochondria! probe, rhodamine123. At that concentration PY is not toxic but suppressed cellgrowth, arresting cells. Pyronin Y has long been used, incombination with other dyes such as Methyl Green, as a differentialstain for nucleic acids in paraffin tissue sections. In sectionsstained with Methyl Green-pyronin Y, red blood cells, elastic fibreof blood vessels, zymogen granules of pancreatic acinar cells,surface membrane of heptocytes and kidney tubular cells showedstrikingly strong green and/or red fluorescence, while the nucleiof cells appeared non-fluorescent. Pyronin Y binds to doublestranded RNA (dsRNA) including messenger RNA (mRNA), transfer RNA(tRNA) and ribosomal RNA (rRNA).危险品标志Xn - 有害物品 Xi - 刺激性物品 风险术语R68 - 可能有不可逆后果的危险。 R36/37/38 - 刺激眼睛、呼吸系统和皮肤。 安全术语S22 - 切勿吸入粉尘。 S24/25 - 避免与皮肤和眼睛接触。 S36 - 穿戴适当的防护服。 S26 - 不慎与眼睛接触后，请立即用大量清水冲洗并征求医生意见。 WGK Germany3RTECSB海关编号32041300
