twistdx-twistamp nfo-tanfo02kit
twistamp nfo
(tanfo02kit)
产品描述：
twistamp® 核酸扩增产品，能够在15分钟内进行常温下的单分子核酸检测该技术对硬件设备的要求很低，特别适合用于体外诊断、兽医、食品安全、生物安全、农业等领域。twistamp nfo用于末端检测，检测dna扩增反应. 末端三明治法快速检测，如侧向流试纸法.除基本组分之外， nfo核酶，和适合是lf 探针 形成新的聚合酶增强底物，无需任何仪器。本品可通过加入exo探针作为amp exo kit的替代物，进行荧光定量检测，由于核酶延缓动态反应速度，可用于末端电泳检测。
basic information:
rpa
1) primers must be 30-35 bases
2) works best at constant temperature (37-39°c)
3) amplicons of 80-400bp are preferred
4) twistamp® lf probe or twistamp® exo probe 1 required - see overleaf
set-up (single-plex)2
1) prepare reaction mix in 1.5ml tube:
primer a (10μm) 2.1 μl
primer b (10μm) 2.1 μl
twistamp® lf probe (10μm) 0.6 μl
rehydration buffer 29.5 μl
template and dh2o 13.2 μl
(total volume 47.5 μl)
vortex and spin briefly
2) add reaction mix to freeze-dried reaction. pipette to mix.
3) add 2.5 μl of 280mm mgac (supplied) and mix well to start reaction.
4) incubate at 37-39°c for 20-40 minutes.
4b) for low template copy number,remove strip after 4 minutes, vortex &spin briefly, replace in heating device.
5) for analysis by lateral flow, dilute reacion products 1/50 with pbst and load 10 μl onto the sample pad of appropriate
strip (eg milenia® hybridetect strips).
6) place strip in pbst running buffer.
warning: rpa reactions start at room temperature as soon as
magnesium is added.
TwistDx